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GMD@CSB.DB: The Analytic Technology Pages
The Analytic@GMD Pages harbours information related to analytical technologies, methods and protocols integrated in GMD as well as and other necessary information related to the field of metabolomics. For in-depth information as well as collaborations we recommended contacting the responsible scientist(s) by the given mail contacts.
To get information of your choice use the links listed below.
Technology
Platform |
Method |
Derivatization |
Extraction |
Through-put (max.) |
Citation |
Group |
| GC-MS Metabolite profiling |
M[1] GC-TOF-MS (EITTMS) |
MEOX TMS |
hot methanol / chloroform |
150-190/ week |
Wagner, C., Sefkow, M., Kopka, J. (2003). Phytochemistry, 62, 887-900. |
Kopka,J. |
| M[2] GC-QUAD-MS (EIQTMS) |
MEOX TMS |
80-100/ week |
|
| M[3] GC-TOF-MS (EITTBS) |
MEOX TBS |
n.d. |
|
| M[4] GC-IONTRAP-MS |
TBS |
hot Bieleski solvent |
10-20/ week |
Birkemeyer, C., Kolasa, A., Kopka, J. (2003). Journal of Chromatography A, 993, 89-102. |
| HPLC |
C18-nucleosyl |
none |
ethylacetate fraction |
n.d. |
|
Gibon,Y. |
| C18-nucleosyl |
none |
methanol fraction |
n.d. |
|
| C18-reverse phase |
OPA |
ethanol |
n.d. |
|
| Ion exchange |
none |
TCA |
n.d. |
|
| reversed phase |
Dansyl |
|
n.d. |
|
Hincha,D. |
| HPAEC-PAD |
Ion exchange |
none |
ethanol |
n.d. |
|
| MTP (Colorimetric assay) |
Ninhydrine |
|
ethanol |
186/ day |
|
Gibon,Y. |
| Bradford |
none |
ethanol |
500-1000/ week |
|
| MTP (Enzyme assay) |
Endpoint |
|
ethanol |
186/ day |
|
| Endpoint + Cycling |
|
ethanol |
186/ day |
Gibon, Y., Vigeolas, H., Tiessen, A., Geigenberger, P., Stitt, M. (2002). Plant Journal, 30, 221-235 |
| Cycling |
|
TCA |
<100/ day |
Gibon, Y., Vigeolas, H., Tiessen, A., Geigenberger, P., Stitt, M. (2002). Plant Journal, 30, 221-235 |
| Endpoint |
|
NaOH |
500-1000/ week |
|
| MTP (Fluorimetric assay) |
|
|
ethanol |
186/ day |
|
| MTP (Spectrophotometric assay) |
|
|
ethanol |
186/ day |
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For suggestions and questions feel free to contact the CSB.DB curator. |
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Update 2005-07-25
A.thaliana: Affymetrix to AGI Assignments updated. New co-response data matrices will be available soon... query data or matrix info.
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Update 2005-07-25
A.thaliana: Affymetrix to AGI Assignments updated. AffymetrixID to AGI code converter will be available soon... query data
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Update 2005-07-25
GMD@CSB.DB - The Golm Metabolome Database: access to custom mass spectra libraries, metabolite profils and related information... get overview |
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Requirements
Minimal resolution for optimal view: 1024x768 (without favorites)! Web browser of the sixth generation required, e.g. MS IE 6.0. Javascript must be enabled... more |
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